EXTRACTION OF DNA WITH THE HELP OF HOUSEHOLD PRODUCTS

 

EXTRACTION OF DNA WITH THE HELP OF HOUSEHOLD PRODUCTS

-      By Nitish Kumar

-      Batch 2k19 , Deptt. Of Chemical Engg.

-      BIT  Sindri , Dhanbad

BRIEF DESCRIPTION ABOUT DNA

DNA stands for Deoxyribose nucleic acid act as a genetic material in most organism. It is a long polymer of deoxyribonucleotides. The Number of nucleotides or base pair present in a DNA determines its Length.

DNA STRUCTRE AND THEIR ELEMENTS

James Watson and Francis Crick in 1953 proposed a Double helix model for DNA structure on the basis of X-ray diffraction.

DNA consist of following elements as follow:

i.          A Pentose Sugar

ii.        A Nitrogenous base

iii.       A Phosphate groups

HOUSEHOLD MATERIALS REQUIRED

a)        Detergent

b)        Common salt

c)        Deodorant(ethanol)

d)        Test Tube or glass

e)        Water

f)         Stop watch

 

                   

                   

PROCEDURE

·        First, take a glass or Test Tube.

·        Now add 80-100 ml water on glass.

·        After that add 8-10gm of common salt on glass

·        Stir it gently and wait for one minute.

·        Now take this solution in mouth for two minutes.

·        Take out this solution from mouth and thus chick cell acquires on solution.

·        Now put it on glass and add 4-5ml ethanol on it.

 

OBSERVATION

Now after 10 to 15 minutes we observed that a white layer start appearing which is DNA of that sample and thus DNA extracted with the help of household products.

SOME INTERESTING FACTS ON DNA

·        Your DNA could stretch from the earth to the sun and back ~600 times.

·       Every human being shares 99.9% of their DNA with every other human.

·        If you could type 60 words per minute, eight hours a day, it would take approximately 50 years to type the human genome.

·        You also share 85% of your DNA with a mouse, 40% with a fruitfly, and 41% with a banana.

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